The 4th RIKEN BRC Training Course for Cryopreservation of Mouse embryos and sperm
	(Oct 10th - 12th, 2006)
Four trainees from research institutes and universities participated in the training course to learn advanced technology for cryopreservation of mouse embryos and sperm. We also lectured and demonstrated intracytoplasmic sperm injection (ICSI) and generation of cloned mouse by nuclear transfer.
<schedule> Day1: Opening ceremony, introduction for facility, lecture (reproductive engineering, in vitro fertilization and cryopreservation of sperm), practical training (preparation for in vitro fertilization and culture media), video instruction (cryopreservation of mouse embryo; lectured by Dr. Edashige from Kochi University) Day2: practical training (cryopreservation of sperm, in vitro fertilization, preparation for freezing solution, fixation and staining of egg, and washing embryo), lecture (cryopreservation, culture and transfer of embryos) Day3: practical training (in vitro fertilization using cryopreserved sperm, examination of fertilization, embryo freezing and thawing) Day4: practical training (examination of fertilization, microscopic observation of embryo, fixation and staining of sperm), lecture and observation (intracytoplasmic sperm injection and nuclear transfer), Qs and As, closing ceremony