EGFP-Akt Transgenic Mouse A model for visualization of PI(3,4,5)P3 dynamics BRC No. 01155_EGFP-Akt-2 transgenic mouse BRC No. 01156_EGFP-Akt-3 transgenic mouse

Research Application : Cancer Research, Cell Biology Research, General Purpose, Metabolism Research, and Immunology and Inflammation Research

BRC No.01156 EGFP-Akt-3 transgenic mouse Concentrated fluorescent activity of AktPH-GFP at the leading edge (Arrow Heads) during chemotactic peptide-stimulated neutrophil migration was visualized by confocal fluorescence microscopy.

PI3Ks (phosphoinositide 3-kinases) regulate many critical cellular responses by producing PI(3,4,5)P3 (phosphatidylinositol 3,4,5-trisphosphate). The EGFP-Akt transgenic mice express a fluorescent bioprobe for PI(3,4,5)P3/PI(3,4)P2 (phosphatidylinositol 3,4-bisphosphate) which is the pleckstrin homology domain of Akt (AktPH) and EGFP fusion protein. The AktPH-GFP fluorescence shows defused localization in cytoplasm, however the fluorescence is concentrated at the leading edge during fMet-Leu-Phe (N-formylmethionyl-leucylphenylalanine; chemotactic peptide)-stimulated neutrophil migration. These transgenic mice are useful for studying PI(3,4,5)P3 metabolism in physiological and pathological situations by crossing your mutant mice.

Depositor : Dr. Takehiko Sasaki (Akita University) (Japanese page) Reference : Nature Cell Biol. (2007) 9(1):36-44. Biochemical Society Transactions (2007) 35(Pt 2):215-8.