BALB/c Hbb Congenic Strains Sustaining Hemoglobin Beta Chain Variations

BALB/c-Hbbp RBRC 00578 BALB/c-Hbbw1 RBRC 00568 BALB/c-Hbbw2   RBRC 01451 BALB/cAn(Hbbd) RBRC 00641

Hbb : Hemoglobin beta chain complex (Chr7)

RBRC No.00641 BALB/cAn (Hbbd) Schematic figures of protein electrophoresis patterns using acetate cellulose plates. Four Hbb haplotypes can be distinguished by iodoacetic acid treatment (Right). Left : Blood samples were treated by distilled water. Right : Blood samples were treated by iodoacetic acid.

Several haplotypes of the hemoglobin beta chain (Hbb) have so far been identified; d and s in the common inbred strains, and p, w1 and w2 in the Asian wild-derived strains [1-4]. The unique Hbb haplotypes derived from wild mice have been introduced into the BALB/c genetic background. Haplotype-p was introduced from the Japanese wild-derived strain MSM/Ms (Mus musculus molossinus), w1 from the mouse captured at Jiayuguang, Gansu Province in China, and w2 from the mouse captured around Lake Balkhash in the Republic of Kazakhstan. These haplotypes can be distinguished by cellulose-acetate membrane electrophoresis. An analysis of nucleotide sequences of Hbb-b1, -b2 and the neighboring genome revealed that the p haplotype is a recombination between d and w1 haplotype [4,5,6]. These congenic strains are invaluable resources to resolve the evolutionary and phylogeographic history of the wild and domesticated populations of M. musculus, as well as the biological functions of hemoglobin.

Depositor : Dr. Toshihiko Shiroishi, National Institute of Genetics Dr.Tetsuo Morita, University of Miyazaki. (HP in Japanease only)

Reference : 1. Minezawa, M. et al. 1979. Geographical distribution of Hbbp allele in the Japanese wild mouse, Mus musculus molossinus. Japan Journal of Genetics. 54: 165-173. 2. Miyashita, N. et al. 1985. Allelic constitution of the hemoglobin beta chain in wild populations of the house mouse, Mus musculus. Biochem Genet. 23: 975-986 3. Kawashima, T. et al. 1991.A new haplotype of the beta-globin gene complex, Hbb-w1, in Chinese wild mouse. Jpn J Genet. 66: 491-500 4. Ueda, Y. et al. 1999. Nucleotide sequences of the mouse globin beta gene cDNAs in a wild derived haplotype Hbb-w1. Mammalian Genome. 10: 879-882 5. Sato, J.J. et al. 2006. Further evidence for recombination between mouse hemoglobin beta b1 and b2 genes based on the nucleotide sequences of intron, UTR, and intergenic spacer regions. Genes Genet Syst. 81: 201-20 6. Sato, J.J. et al. 2008. Discovery of a new HBB haplotype w2 in a wild-derived house mouse, Mus musculus. Mamm Genome. 19: 155-162.