Visualizing the Wnt signal pathway

TOP-GAL RBRC 02228 TOP-EGFP RBRC 02229

Visualization of the Wnt canonical signaling pathways in (A) the TOP-GAL (RBRC02228) by X-gal staining and (B) the TOP-EGFP (RBRC02229) by green fluorescence at embryonic days 10.5.

Wnt proteins belong to a family of secreted and lipid-modified glycoprotein that can activate intracellular signaling pathways, such as the Wnt/planar cell polarity, the Wnt/calcium and the Wnt β-catenin or so-called Wnt canonical signaling pathways. The Wnt canonical pathway during embryogenesis has been intensively studied for past years. However, the role of this pathway in the neonatal and adult tissues is unknown. The TOP-GAL (RBRC02228) and TOP-EGFP (RBRC02229) transgenic mice are reporter lines which can visualize the Wnt canonical pathway by β-galactosidase activity and green fluorescence in adult tissues, respectively. The two transgenic mice were generated with ins-TOPGAL and ins-TOPEGFP that accurately drive β-galactosidase and EGFP expression under TCF/β-catenin. These transgenic lines will provide you unique opportunity to investigate the function of Wnt canonical pathway in vivo.

Depositor : Professor Yumiko Saga, National Institute of Genetics, Mishima, Japan Reference : 1. Genesis. 2007 Feb;45(2):90-100.