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Mouse iPS cell lines (APS)
Animal ES cell lines including nuclear transferred ES cell lines and germline stem cell lines (AES)
Cell lines derived from human and other animals (RCB)
EBV transformed B cell lines derived from Japanese (HEV)
Sonoda-Tajima Collection: EBV-transformed B cell lines derived from various human species (HSC)
Goto Collection:EBV transformed B cell lines and primary fibroblasts derived from Werner syndrome patients (GMC)
Cord Blood Stem Cells (HCB,C34)
Mesenchymal Stem Cells (HMS)
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 Mouse iPS cell lines (APS)
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Kyoto University simplified the distribution procedure for cell lines.
 You no longer have to execute a separate agreement with Kyoto University.
 CiRA , Kyoto University
     http://www.cira.kyoto-u.ac.jp/e/index.html
Available cell line
Depositor : Dr. Shinya Yamanaka, Kyoto University, Japan
Cell line : APS0001  iPS-MEF-Ng-20D-17
Nature 448: 313-317 (2007), Nature Protocols 2: 3081-3089 (2007)
The culture method used in RIKEN BRC:
Medium : DMEM (high glucose, without Sodium pyruvate)
+ 15% FBS
+ 0.1 mM  NEAA
+ 0.1 mM  2-Mercaptoethanol
+ 1000 U/ml  mouse LIF
Feeder cells : Mouse embryonic fibroblasts (MEF)
New cell lines
    APS0002  iPS-MEF-Ng-178B-5
        Nature Biotechnology 26 : 101-106 (2008)
    APS0003  iPS-MEF-FB/NG-440A-3
        Science 322 : 949-953(2008)
    APS0004  iPS-MEF-Ng-492B-4
        Science 322 : 949-953(2008)
The culture method used in RIKEN BRC:
Medium : DMEM (high glucose, without Sodium pyruvate)
+ 15% FBS
+ 0.1 mM  NEAA
+ 0.1 mM  2-Mercaptoethanol
+ 1000 U/ml  mouse LIF
Feeder cells : SNLcell line (SNL 76/7)
 CiRA , Kyoto University
     http://www.cira.kyoto-u.ac.jp/e/pressrelease/news/091001-090000.html
Procedures to use:
1. The user should send the following two Forms to the RIKEN BioResource Center (RIKEN BRC).
(1)"Order Form (C-0033)"
(2)"MTA between the RIKEN BRC and the user (C-0034)"; two sets.
* The cell lines available from the RIKEN BRC Cell Bank are listed on the website and catalog.
Please print "TERMS and CONDITIONS" and attach it to MTA(C-0034).
  Cell Search http://www2.brc.riken.jp/lab/cell/search.php
     Order Form
Mailing address is as follows:
Cell Bank, RIKEN BioResource Center
Koyadai 3-1-1, Tsukuba, Ibaraki, 305-0074, Japan
If you had any question, please e-mail to the following address.
cellqa@brc.riken.jp
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Cell Bank, RIKEN BioResource Center
3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan
FAX : +81-29-836-9130 / E-mail : cellbank@brc.riken.jp


RIKEN Bio Resource Center Cell Bank