

|
| Mouse iPS cell lines (APS) |
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| Kyoto University simplified the distribution procedure for cell lines. |
| You no longer have to execute a separate agreement with Kyoto University. |
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| CiRA , Kyoto University |
| http://www.cira.kyoto-u.ac.jp/e/index.html |
| Available cell line |
| Depositor |
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Dr. Shinya Yamanaka, Kyoto University, Japan |
| Cell line |
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APS0001 iPS-MEF-Ng-20D-17 |
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Nature 448: 313-317 (2007), Nature Protocols 2: 3081-3089 (2007) |
| The culture method used in RIKEN BRC: |
| Medium |
: |
DMEM (high glucose, without Sodium pyruvate)
+ 15% FBS
+ 0.1 mM NEAA
+ 0.1 mM 2-Mercaptoethanol
+ 1000 U/ml mouse LIF |
| Feeder cells |
: |
Mouse embryonic fibroblasts (MEF) |
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| New cell lines |
| APS0002 iPS-MEF-Ng-178B-5 |
| Nature Biotechnology 26 : 101-106 (2008) |
| APS0003 iPS-MEF-FB/NG-440A-3 |
| Science 322 : 949-953(2008) |
| APS0004 iPS-MEF-Ng-492B-4 |
| Science 322 : 949-953(2008) |
| The culture method used in RIKEN BRC: |
| Medium |
: |
DMEM (high glucose, without Sodium pyruvate)
+ 15% FBS
+ 0.1 mM NEAA
+ 0.1 mM 2-Mercaptoethanol
+ 1000 U/ml mouse LIF |
| Feeder cells |
: |
SNLcell line (SNL 76/7) |
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| CiRA , Kyoto University |
| http://www.cira.kyoto-u.ac.jp/e/pressrelease/news/091001-090000.html |
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| Procedures to use: |
| 1. |
The user should send the following two Forms to the RIKEN BioResource Center (RIKEN BRC).
(1)"Order Form (C-0033)"
(2)"MTA between the RIKEN BRC and the user (C-0034)"; two sets. |
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* |
The cell lines available from the RIKEN BRC Cell Bank are listed on the website and catalog.
Please print "TERMS and CONDITIONS" and attach it to MTA(C-0034).
Cell Search http://www2.brc.riken.jp/lab/cell/search.php |
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| Order Form |
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| Mailing address is as follows: |
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Cell Bank, RIKEN BioResource Center
Koyadai 3-1-1, Tsukuba, Ibaraki, 305-0074, Japan |
If you had any question, please e-mail to the following address.
cellqa@brc.riken.jp
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Cell Bank, RIKEN BioResource Center
3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan
FAX : +81-29-836-9130 / E-mail : cellbank@brc.riken.jp |
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