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Genomic DNA

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JCM Microbial Genomic DNA

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General Information

Bacteria and archaeans are cultured in the RIKEN BRC-JCM and their DNA are prepared in the Gene Engineering Division. High molecular weight DNA is prepared by phenol-chloroform extraction. The DNA is suitable for amplification by PCR. The concentration and purity of DNA samples are determined spectrophotometrically and further checked by PCR amplification of 16S rRNA gene and following electrophoresis. Nucleotide sequences are also confirmed. DNA concentrations generally range from 50 to 100 ng/μl. (The concentrations of individual samples are supplied with a data sheet.) The DNAs are stored in TE buffer (10 mM TRIS pH7.5, 1mM EDTA) at -20oC.

Attention International Transfer - "Veterinary Permission" or "Permit to Import Quarantine Material" may be required for shipment of the biological materials. Please follow guide lines in your country.

Forms for Distribution


    Ordering information is at http://dna.brc.riken.jp/en/furnish.html

Forms required

Forms Description
Form A Order Form
Please specify strain names with RDB numbers (catalog num.).
Form C Material Transfer Agreement
We ask a signature of the Authorized Representative by an administrator of intellectual property.
Please put in the terms and condisions that
"The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication."
(Please e-mail to dnabank@brc.riken.jp to obtain MTA in MS-Word format)
    Please obtain order forms available at http://dna.brc.riken.jp/en/furnish.html

    If you are inquiring about the availability of a specific DNA, please give as much information about the strain stock number as possible.
    For more information contact:
    [Regarding DNA] [Regarding bacteria strains]
    Gene Engineering Division,
    RIKEN BioResource Center
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    Fax: +81-29-836-9120
    dnabank@brc.riken.jp
    		 Microbe Division / Japan Collection of Microorganisms
    RIKEN BioResource Center
    2-1 Hirosawa, Wako, Saitama 351-0198, Japan
    Fax: +81 48 462 4617
    inquiry@jcm.riken.jp

    The availability of some DNAs is limited. All requests for these DNAs will be given due consideration, but we cannot assure availability.

Personal data protection policy
For protection of personal data at RIKEN BioResource Center, please reffer Personal data protection policy.

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2009.09.07