JCM DNA

BRC-JCM Bacterial Genomic DNA

General Information

Bacteria are cultured in the RIKEN BRC-JCM and their DNA are prepared in the Gene Engineering Division. High molecular weight DNA is prepared by phenol-chloroform extraction. The DNA is suitable for amplification by PCR. The concentration and purity of DNA samples are determined spectrophotometrically and further checked by PCR amplification of 16S rRNA gene and following electrophoresis. Nucleotide sequences are also confirmed. DNA concentrations generally range from 50 to 100 ng/μl. (The concentrations of individual samples are supplied with a data sheet.) The DNAs are stored in TE buffer (10 mM TRIS pH7.5, 1mM EDTA) at -20^oC.

Attention International Transfer - "Veterinary Permission" or "Permit to Import Quarantine Material" may be required for shipment of the biological materials. Please follow guide lines in your country.

Forms for Distribution

Ordering information is at http://www.brc.riken.jp/lab/dna/en/furnish.html

Forms required

Ordering information is at http://www.brc.riken.jp/lab/dna/en/furnish.html

If you are inquiring about the availability of a specific DNA, please give as much information about the strain stock number as possible.
For more information contact:


[Regarding DNA]	[Regarding bacteria strains]
Gene Engineering Division, RIKEN BioResource Center 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan Fax: +81-29-836-9120 dnabank@brc.riken.jp	Microbe Division / Japan Collection of Microorganisms RIKEN BioResource Center 2-1 Hirosawa, Wako, Saitama 351-0198, Japan Fax: +81 48 462 4617 inquiry@jcm.riken.jp


The availability of some DNAs is limited. All requests for these DNAs will be given due consideration, but we cannot assure availability.