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> Vector (ja)
> Shuttle-vector system for S. cerevisiae
Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins
Living modified organisms (LMO)
Laboratory use only.
Not intended for intentional introduction into the environment.
| Marker | Name | Size (kb) | Usable sites | RDB no. |
|---|---|---|---|---|
| TRP1 | pAMT10 | 4.3 | 5 | 1951 |
| TRP1 | pAMT20 | 5.1 | 5 | 1952 |
| TRP1 | pGMT10 | 4.6 | 5 | 1959 |
| TRP1 | pGMT20 | 5.4 | 5 | 1960 |
| HIS3 | pAMH10 | 4.5 | 6 | 1947 |
| HIS3 | pAMH20 | 5.3 | 6 | 1948 |
| HIS3 | pGMH10 | 4.8 | 6 | 1955 |
| HIS3 | pGMH20 | 5.6 | 6 | 1956 |
| LEU2 | pAML10 | 5.1 | 6 | 1949 |
| LEU2 | pAML20 | 5.9 | 6 | 1950 |
| LEU2 | pGML10 | 5.4 | 6 | 1957 |
| LEU2 | pGML20 | 6.2 | 6 | 1958 |
| URA3 | pAMU10 | 4.6 | 6 | 1953 |
| URA3 | pAMU20 | 5.4 | 6 | 1954 |
| URA3 | pGMU10 | 4.9 | 6 | 1961 |
| URA3 | pGMU20 | 5.7 | 6 | 1962 |
| RDB no. | Name | Running title | Type | Tag | Ori | Marker | Promoter |
|---|---|---|---|---|---|---|---|
| 1947 | pAMH10 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | centromere-type | HIS3 | ADH1 |
| 1948 | pAMH20 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | 2micron | HIS3 | ADH1 |
| 1949 | pAML10 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | centromere-type | LEU2 | ADH1 |
| 1950 | pAML20 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | 2micron | LEU2 | ADH1 |
| 1951 | pAMT10 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | centromere-type | TRP1 | ADH1 |
| 1952 | pAMT20 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | 2micron | TRP1 | ADH1 |
| 1953 | pAMU10 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | centromere-type | URA3 | ADH1 |
| 1954 | pAMU20 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | 2micron | URA3 | ADH1 |
| 1955 | pGMH10 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | centromere-type | HIS3 | GAL1-10 |
| 1956 | pGMH20 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | 2micron | HIS3 | GAL1-10 |
| 1957 | pGML10 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | centromere-type | LEU2 | GAL1-10 |
| 1958 | pGML20 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | 2micron | LEU2 | GAL1-10 |
| 1959 | pGMT10 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | centromere-type | TRP1 | GAL1-10 |
| 1960 | pGMT20 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | 2micron | TRP1 | GAL1-10 |
| 1961 | pGMU10 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | centromere-type | URA3 | GAL1-10 |
| 1962 | pGMU20 | E. coli - S. cerevisiae shuttle vector | Vector | Myc | 2micron | URA3 | GAL1-10 |
Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.
Depositor: Dr. Hidekatsu Iha
Reference: Biotechniques 25 (6): 936-938, 1998.
National BioResource Project - Yeast
Strains and plasmids of budding yeast (Saccharomyces cerevisiae) is available.
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