Depositors List is up dated (2010.09.08)

♦ Please visit our Depositors List of Genetic Materials in the Gene Engineering Division.

Report on your achievements is appreciated (2010.07.07)

♦ We appreciate the report on your achievements such as research papers and/or patents which were obtained though use of our DNA materials. Your report will be added immediately to the accompanying information of the DNA material. This is an excellent way to increase the utility value of the DNA material as well as the visibility of your research.
♦ Please send us the following information to dnabank@brc.riken.jp:
1) Research Paper: First Author, Title, Name of the Journal, Volume, Pages and Published Year
2) Patent: Holder, Title, Number and Year
♦ Your publication and patent information will be added to the accompanying information of the bioresource in web catalog and to the list of publication.
http://www.brc.riken.jp/lab/dna/ja/reflist.html.
♦ When you publish your research results in a scientific journal, please refer to the origin of materials in Materials & Methods or Acknowledge section as follows:
________ (name of BIOLOGICAL RESOURCE) was provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan.
♦ Your cooperation and understanding are essential for enrichment of information on bioresource, improvement of quality of scientific research and sustainable operation of our Center.

Genome Network Project Human Full-Length cDNA Clone is now available (2010.04.20)

♦ The RIKEN BioResource Center is distributing copies of full-length human cDNA clones, produced and organized through the MEXT Genome Network Project to academia under its distribution policy.
♦ There are two types of cDNA clone collections.
1) Human Full-Length cDNA clones
Approximately 30,000 clones corresponding to 60% of all human genes (14,000 genes) produced and organized by Dr. Sumio Sugano of the University of Tokyo and Dr. Yoshihide Hayashizaki of RIKEN OSC.
2) Human Gateway® Entry clones
Approximately 50,000 clones corresponding to 6,300 genes produced by cloning PCR-amplified open reading frame fragments from full-length human cDNA clones and studied through the government-supported MEXT Genome Network Project. DNA fragments cloned into Gateway® entry clones can be transferred into one or more destination vectors simultaneously using Gateway® technology. The Gateway® Entry clones include clones made from cDNA created by the Research Association for Biotechnology under the New Energy and Industrial Technology Development Organization Full-length Human cDNA Sequencing Project.
♦ Results of the project attained using the Genome Network Project cDNA clones have appeared in journals including Nature Genetics. Detailed information on these clones is available via the Genome Network Platform (http://genomenetwork.nig.ac.jp/).
More about Genome Network Project Human Full-Length cDNA Clone

C57BL/6N (B6N) Mouse BAC clone is now available (2010.03.01)

♦ The Gene Engineering Division has constructed a BAC library of the B6N substrain and the RIKEN BioResource Center completed BAC end sequencing of B6N and register the sequence data with DDBJ in a collaboration with the National Institute of Genetics under the Genome Information Upgrading Program of MEXT NBRP. The B6N Mouse BAC library consisted of 62,000 clones representing an estimated coverage of 2.6-fold and 77% haploid genome.
♦ A new database, 'Mouse BAC Brower' is also available at the site of the National Institute of Genetics (http://analysis2.lab.nig.ac.jp/mouseBrowser/). This database provides information of genomic locations of the B6N Mouse BAC clones on a B6J's genome sequence as well as the context of other features, such as genes. Using the database, researchers can be find BAC clones in silico with direct querying (ex. gene symbol, name of gene product, gene ID and so on).
More about B6N BAC clone
♦ If you are interested in our BAC clones, please feel free to contact us.
♦ The germ cell competent C57BL/6N ES cell lines are available from the Cell Engineering Division of RIKEN BRC.
http://www.brc.riken.jp/lab/animal/mailnews/nm200909_02.html

Addition of data of immunofluorescent staining for the full CDS expression clones (2010.02.19)

♦ The Gene Engineering Division provides full CDS expression clones that were constructed by our division, and the confirmed entire nucleotide sequences of each clone and their expression proteins determined by the western blotting and the immunofluorescent staining.
♦ We opened additional data of immunofluorescent staining for cells introduced expression vectors listed below.
♦ If you are interested in our BAC clones, please feel free to contact us.
More about pCMV_S-FLAG expression clones
List of genes
RDB 6068 human PPARD/PPAR delta
RDB 6069 human ROR beta
RDB 6070 human TR beta; ErbA-beta
RDB 6108 human CREB3
RDB 6111 human NFE2
RDB 6295 human ER beta
RDB 6598 human Oct3/4 isoform1
RDB 6600 human Sox2
RDB 6601 human Nanog homeobox
RDB 6602 human LIN28
RDB 6670 human Klf4
RDB 6671 human c-Myc
RDB 6998 human IL6
RDB 6999 human CPSF5/CPSF25
RDB 7012 human CREB2; ATF4
RDB 7013 human NRF1; NFE2L1
RDB 7014 human NRF1; NFE2L1
RDB 7015 human NRF3; NFE2L3
RDB 7016 human E2F8
RDB 7017 human NFKB2
RDB 7018 human NFKB1
RDB 7019 human SMAD3
RDB 7020 human Sap-1
RDB 7026 human MYCL1, transcript variant 3
RDB 7027 human neural cell adhesion molecule 1, transcript variant 1
RDB 7028 human AKT2
RDB 7029 human v-myc myelocytomatosis viral oncogene homolog (avian)
RDB 7270 human PEA3
RDB 7271 human SRY(sex determining region Y)-box 11

BAC Brower of Japanese macaque is released (2010.02.01)

♦ RIKEN DNA Bank is distributing BAC clones of Japanese macaque (Macaca fuscata fuscata).
♦ A new database, 'BAC Brower of Japanese macaque' is constructed by the Information Center of the National BioResource Project, National Institute of Genetics and now available (http://analysis2.lab.nig.ac.jp/jmonkeyBrowser). This database provides information of genomic locations of the Japanese macaque BAC clones on a rhesus macaque's genome sequence as well as the context of other features, such as genes.
♦ Using the database, researchers can be find BAC clones in silico with direct querying (ex. gene symbol, name of gene product, gene ID and so on).
♦ If you are interested in our BAC clones, please feel free to contact us.
More about Japanese macaque BAC clone

Genomic DNAs form the culture collection of RIKEN BRC-JCM is available (2010.01.28)

♦ The RIKEN DNA Bank provides genomic DNAs prepared from microorganisms of the RIKEN BRC-JCM. The strains listed below are newly available.
Bifidobacterium longum subsp. infantis JCM 1222^T
Clostridium paraputrificum JCM 1293^T
Clostridium pasteurianum JCM 1408^T
Corynebacterium glutamicum JCM 1318^T
Corynebacterium kroppenstedtii JCM 11950^T
Lactobacillus acidophilus JCM 1132^T
Lactobacillus gasseri JCM 1131^T
Methanocaldococcus fervens JCM 15782^T
Methanocaldococcus infernus JCM 15783^T
Methanopyrus kandleri JCM 9639^T
Mycobacterium kansasii JCM 6379^T
Nocardia asteroides JCM 3384^T
Propionibacterium acnes JCM 6425^T
Streptococcus oralis JCM 12997^T
Streptococcus salivarius subsp. thermophilus JCM 20026
More about Microorganisms genomic DNA

New Entry of Promoter Collection (2009.12.08)

♦ The promoter, one of the most important regions of the gene to regulate the transcription has been collected as a large series in the DNA Bank, RIKEN BioResource Center. These promoters were subcloned into plasmid that has a firefly luciferase reporter gene as a promoter reporter construct (the Promoter Series).
♦ We opened and started distributing of the novel 108 promoter constructs (80 types of TP53 targeted genes and 28 types of ATF family targeted genes). All of the Promoter series were validated by restriction enzyme digestion and confirmation of end sequences of the insert DNA. Furthermore, we checked the promoter activities of the Promoter series on 3 kinds of cell lines (HeLa, HepG2 and Hep3B cells).
♦ The Promoter Series are very useful for individual and/or comprehensive analysis of transcriptional regulation of genes. And each construct is also available for a source of tissue specific expression of genes that you focused.
More about Promoter Collection

Life Technologies Corporation signs license agreement with RIKEN BioResource Center (2009.11.03)

♦ The Life Technologies Corporation (former Invitrogen IP Holdings, Inc.) and RIKEN BioResource Center have signed a license agreement on July 2, 2009. With generosity of Life Technologies Corporation, Riken BRC is now able to receive, maintain, replicate and distribute Gateway® Entry clones and Expression clones for not-for-profit academic research under this agreement.
♦ The Entry clone harboring cDNA can be distributed for not-for-profit academic research without any license fees. The Expression clone harboring mammalian cDNA can be distributed from the RIKEN BRC for not-for-profit academic research with a small amount of license fee to the Life Technologies Corporation. In addition to the regular fee for a cDNA clone, users are asked to pay RIKEN BRC 1,250 YEN per clone that includes the license fee, handling of currency exchange, money transfer and other expense. Every six months, RIKEN BRC will report to Life Technologies Corporation a number of distributions of Entry and Expression clones and send a sum of license fee for Expression clones. Any commercial users of the Gateway® Entry or Expression clones must contact Life Technologies Corporation for prior written approval.
♦ RIKEN BRC has set the Material Transfer Agreements (MTA) for each transfer to protect the intellectual property rights of developers of bioresources and to define the responsibility of users. We have opened a path of the not-for-profit academic use of bioresources produced by using research tools owned by commercial entities.
♦ Deposition of bioresources to the RIKEN BRC frees researchers from cumbersome preservation and distribution of materials fellow researchers. Furthermore, deposition increases chance of collaboration and citation of research paper. Your deposition of genetic resources in particular the Gateway® clones to the Gene Engineering Division is most appreciated.

Genomic DNAs form the culture collection of RIKEN BRC-JCM is available (2009.11.03)

♦ The RIKEN DNA Bank provides genomic DNAs prepared from microorganisms of the RIKEN BRC-JCM. The strains listed below are newly available.
Alcaligenes faecalis JCM 20666
Azotobacter vinelandii JCM 21475^T
Bacteroides thetaiotaomicron JCM 5827^T
Bacillus cereus JCM 20266
Bifidobacterium adolescentis JCM 1275^T
Bifidobacterium catenulatum JCM 1194^T
Blautia hansenii JCM 14655^T
Blautia producta JCM 1471^T
Caldanaerobacter subterraneus subsp. tengcongensis JCM 11007^T
Enterococcus faecalis JCM 20313^T
Geobacillus stearothermophilus JCM 14450
Neisseria mucosa JCM 12992^T
Rhizobium radiobacter JCM 21034
Streptomyces coelicolor JCM 4357^T
More about Microorganisms genomic DNA

The "Whole Cell Project Database" was updated (2009.07.06)

♦ RIKEN DNA Bank is distributing gene disruption plasmids and expression plasmids of Thermus thermophilus HB8 deposited by Dr. Seiki Kuramitsu, the Whole Cell Project, the SR Systembiology Reserch Group, RIKEN SPring-8 Center.
♦ The Whole Cell Project Database has been maintained by the SR Systembiology Reserch Group, RIKEN SPring-8 Center and provided information with the clones for expression of genes of Thermus thermophilus HB8 (http://www.srg.harima.riken.jp/h_db/index.html).
♦ A new version of the database is now available and researchers can be find plasmids for disruption of genes of T. thermophilus HB8.
♦ The gene disruptant of T. thermophils HB8 can be easily prepared by adding this plasmid into the culture medium. The target gene in this plasmid was replaced by the thermostable kanamycine resistant gene. The length of the homologous region outside of the target gene is about 500 bp (only 10 bp of the target gene are left in both sides).
More about Disruption Plasmid of Thermus thermophilus HB8

Genomic DNAs form the culture collection of RIKEN BRC-JCM is available (2009.07.06)

♦ The RIKEN DNA Bank is distributing genomic DNAs prepared from microorganisms of the RIKEN BRC-JCM. The strains listed below are newly available.
Pseudomonas aeruginosa JCM 20301
Staphylococcus aureus subsp. aureus JCM 20624^T
More about Microorganisms genomic DNA

Genomic DNAs form the culture collection of RIKEN BRC-JCM is available (2009.06.11)

♦ The RIKEN DNA Bank is distributing genomic DNAs prepared from microorganisms of the RIKEN BRC-JCM. The strains listed below are newly available.
Geobacillus stearothermophilus JCM 14450
Caldanaerobacter subterraneus subsp. tengcongensis JCM 11007^T
Alcaligenes faecalis JCM 20666
Azotobacter vinelandii JCM 21475^T
Bacillus cereus JCM 20266
Streptomyces coelicolor JCM 4357^T
More about Microorganisms genomic DNA

F344 & LE BAC browser is available (2009.04.07)

♦ Rattus norvegicus database F344/Stm and LE/Stm BAC browser is released by NBRP Rat.
Using this database, you may find your BAC clones.
 NBRP Rat BAC Clone

Discount Service for Plasmid, Cosmid and Host strains (2009.04.01)

♦ For large orders of the Plasmid, Cosmid and Host strains, there is a discount depending on the total number of tubes ordered. For single orders of 20 or more tubes, the total charge will be reduced according to the number of tubes.
More about Discount Service for Plasmid, Cosmid and Host strains

FUCCI mice for visualizing cell-cycle in vivo (2009.03.30)

♦ RIKEN and Amalgaam Inc. concluded a bio-genetic resource deposit agreement for launching a delivery service for Fucci mice which are transformed by a fluorescent cell cycle probe.
For detail, visit E-MAIL News No.57
by the Experimental Animal Division, RIKEN BioResource Center.

A mouse iPS cell line is now available (2009.03.28)

♦ Kyoto University, in association with RIKEN, start a program for distribution of mouse iPS cells.
More about The Cell Bank, RIKEN BioResource Center