NIA Mouse cDNA Clone

Complete plate sets and individual clones are available for this resource.

NIA/NIH Mouse 7.4K cDNA Clone Set

This mouse cDNA clone set was prepared by Dr Minoru Ko and colleagues (VanBuren V, et al. Genome Research 2002 Dec;12(12):1999-2003) at the National Institute on Aging which is one of the 25 institutes and centers of the National Institutes of Health.

Further details of this clone set can be obtained from
http://lgsun.grc.nia.nih.gov/cDNA/NIA_7_4k.html.

The set has the following characteristics:

• 7,404 cDNA clones with no redundancy within the set or with NIA Mouse 15k.
  
• This set includes cDNA from the following developmental stage of C57BL/6J mouse
  
  • unfertilized egg (long)
    
  • preimplantation embryo (regular)
    
  • E3.5 brastocyst (long)
    
  • E7.5 embryo (regular)
    
  • E7.5 ectoplacental cone (regular)
    
  • E7.5 extraembryonic part cDNA library
    
  • E7.5 whole embryo (long)
    
  • E12.5 male mesonephros/gonad (long)
    
  • E12.5 female mesonephros/gonad (regular)
    
  • E13.5 VMB dopamine cell (long)
    
  • newborn ovary (regular)
    
  • newborn heart (regular)
    
  • newborn kidney (regular and long)
    
  • newborn brain (regular)
    
  • adult germinal center B cell (regular)
    
  • embryonic stem cell (regular and long)
    
  • hematopoietic stem cell (long)
    
  • mesenchymal stem cell (long)
    
  • trophoblast stem cell cDNA libraries (regular and long)
    (VanBuren et al., 2002, Genome Research Vol. 12, Issue 12, 1999-2003, PMID: 12466305)
    
• About 1.5 kb average insert size for short insert clones and ca. 2.5-4.0kb for longer full length clones.
  
• Clones were sequenced from both 5' and 3' termini to obtain longer reads and verify sequence. Sequence information is available from their web site
  http://lgsun.grc.nia.nih.gov/cDNA/NIA_7_4k.html
  
• This set consists of plates numbered from H4001 -H4079
  
  To see agarose gel images of digested and undigested clons from each plates please click here.
  
  

NIA/NIH Mouse 15K cDNA Clone Set

This mouse cDNA clone set was prepared by Dr Minoru Ko and colleagues (Tanaka et al., PNAS 97, 9127-9132) at the National Institute on Aging which is one of the 25 institutes and centers of the National Institutes of Health.

Full details of this clone set can be obtained from
http://lgsun.grc.nia.nih.gov/cDNA/15k.html.
The clone set has now been distributed widely and is currently in use by a number of microarray programmes in many countries.

The set has the following characteristics:

• About 15,000 'unique' cDNA clones were rearrayed from 52,374 ESTs from pre-and periimplantation embryos, E12.5 female gonad/mesonephros, and newborn ovary as follows.
  
  • preimplantation embryos: unfertilized eggs
    
  • preimplantation embryos: fertilized eggs
    
  • preimplantation embryos: 2-cell embryos
    
  • preimplantation embryos: 4-cell embryos
    
  • preimplantation embryos: 8-cell embryos
    
  • preimplantation embryos: 16-cell embryos
    
  • preimplantation embryos: E3.5 blastocysts
    
  • E7.5 extraembryonic
    
  • E7.5 embryonic
    
  • E12.5 female gonad/mesonephros
    
  • newborn ovary
    (Tanaka et al., 2000, Proc Natl Acad Sci U S A, 97: 9127-9132, PMID: 10922068)
    
• Up to 50% are derived from novel genes.
  
• About 1.5 kb average insert size.
  
• Clones were sequenced from 5' and 3' termini to obtain longer reads and verify sequence. Sequence information is available from their web site
  (http://lgsun.grc.nia.nih.gov/cDNA/15k.html).
  
  
• 95% of the wells produce PCR products with 94% of the products comprising a single amplification band.
  
  To see agarose gel images of the PCR products from each of the 159 plates please click here.
  

References

Web Page

http://lgsun.grc.nia.nih.gov/

Original Papers
NIA mouse 15K cDNA clone set

Tanaka TS, Jaradat SA, Lim MK, Kargul GJ, Wang X, Grahovac MJ, Pantano S, Sano Y, Piao Y, Nagaraja R, Doi H, Wood WH 3rd, Becker KG, Ko MS (2000) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray. Proc. Natl. Acad. Sci. USA, 97: 9127-9132. [PMID:10922068]

Kargul GJ, Dudekula DB, Qian Y, Lim MK, Jaradat SA, Tanaka TS, Carter MG, Ko MS (2001) Verification and initial annotation of the NIA mouse 15K cDNA clone set. Nature Genetics, 28:17-18. [PMID:11326268]

VanBuren V, Piao Y, Dudekula DB, Qian Y, Carter MG, Martin PR, Stagg CA, Bassey UC, Aiba K, Hamatani T, Kargul GJ, Luo AG, Kelso J, Hide W, Ko MS (2002) Assembly, Verification, and Initial Annotation of the NIA Mouse 7.4K cDNA Clone Set. Genome Res 2: 1999-2003. [PMID:12466305]