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Promoter Collection

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  Promoter Construct (ja)   Promoter Construct (en)   Firefly Luc   Renilla Luc
  Promoter Activity   Promoter List

Renilla Luciferase Construct

List of cells

CellsOrganismDescription
B16mouseSkin, melanoma
C2C12mouseMuscle, myoblast
CHOhamstar, ChineseOvary
COS-7monkey, African greenKidney, SV40 transformed, T antigen expressing, derived from CV-1
F9mouseEmbryonal carcinoma
HEK293humanKidney, transformed with adenovirus 5 DNA
HeLahumanCervix, epithelial, adenocarcinoma
HepG2humanLiver, hepatocellular carcinoma
K562humanErythro leukemia, bone marrow, chronic myelogenous leukemia (CML)
LNCaPhumanProstate, metastatic site, left supraclavicular lymph nodecarcinoma
MCF-7humanMammary gland, brest, epithelial, metastatic site, pleural effusion adenocarcinoma
MIN7mouseInsulinoma (Dr. Miyazaki, Osaka Univ., Grad. Sch. Med.)
MtT/SMratPituitary (growth hormone, prolactine producing)
NEC8humanEmbryonal carcinoma, testis
NIH3T3mouseNormal fibroblast
PC12ratAdrenal gland, pheochromocytoma
SaOS-2humanBone, osteocarcoma
WI-38humanLung, fibroblast, normal

List of promoter activity

-, less than 2 compared with empty pKM2L vector (RDB 4026)
+, 2 to 5
++, 5 to 10
actual value, more than 10

Hormone
 human promoterHeLaHepG2COS-7C2C12C2C12 +DMPC12 PC12 +NGFCHO-K1NEC8LNCaPLNCaP +DexB16MCF-7MtT/SMMIN7Saos-2HEK293
5466activin beta-A-----  -+--- + --
5524glycoprotein hormone alpha-subunit -+           16.7   
5889insulin--            +  
5905growth hormone+++           +   
5475bone morphogenetic protein 5-+-         -    
5474bone morphogenetic protein 6--   ++-   -     
5514midkine-++     +   ++++  - 



Viscera
RDBhuman promoterHeLaHeLa +20% FCSHepG2HepG2 +dexamethasoneHepG2 +CoCl2HepG2 heat shockCOS-7C2C12C2C12 +DMC2C12 +2% horse serumPC12PC12 +NGFCHO-K1NEC8LNCaPLNCaP +DexB16MCF-7MtT/SMF9F9 +RASaos-2HEK293K562K562 +TPAWI38
5465alpha-1-antitrypsin- 388.3549.6 32.4--- --    -- --     
5466activin beta-A- -   ---   -+--- +  --   
5467aldose reductase+ ++             +    --  -
5475bone morphogenetic protein 5- +   -          -        
5484elongation factor 1 A-2- -   -+- 11.0 ++              
5505pyruvate dehydrogenase kinase isoform 4- ++  +25.514.6                 
5506plasminogen activator inhibitor 1- 55.8                       
5513ornithine aminotransferase- ++                       
5519inhibitor of differentiation-237.343.4125.2151.0  13.6++++-++33.923.5     ++     65.0 44.2 
5523hypoxia-inducible factor 1 alpha subunit- + -                     
5532alpha-fetoprotein- -++                 -    
5896Brachyury/T protein- -   -                +--
5897SRY-box 17- -   +     +      -- -   
5898Paired box gene 6- -   ++                +++ 



Muscle
RDBhuman promoterHeLaHeLa +20% FCSHepG2HepG2 +dexamethasoneHepG2 heat shockCOS-7C2C12C2C12 +DMC2C12 +2% horse serumPC12PC12 +NGFB16MCF-7K562K562 +TPANIH3T3
5478collagenase-1- +   33.2    11.3 ++++45.4
5484elongation factor 1 A-2- -  -+- 11.0 ++     
5882hexokinase II- -   ++ ++       
5505pyruvate dehydrogenase kinase isoform 4- ++ +25.514.6        
5519inhibitor of differentiation-237.343.4125.2151.0 13.6++++-++33.923.5 ++65.0 44.2 
5535myosin phosphatase target subunit 1++ 69.5   24.8-28.8       
5900Sus scrofa myosin regulatory light chain ventricular isoform- -   + +       
5901cardiac beta-myosin heavy chain- +   -         



Epithelium
RDBhuman promoterHeLaHepG2HepG2 +dexamethasoneHepG2 heat shockCOS-7C2C12C2C12 +DMPC12PC12 +NGFCHO-K1NEC8LNCaPLNCaP +DexB16MCF-7MtT/SMF9F9 +RASaos-2HEK293K562K562 +TPANIH3T3
5466activin beta-A--  ---  -+--- +  --   
5474bone morphogenetic protein 6--     ++-   -         
5478collagenase-1-+   33.2       11.3     ++++ 45.4
5479collagenase type IV-44.5   +-      +-   -   46.9
5480prostaglandin synthase-2/cyclooxygenase +-         +-+         
5492DOPAchrome tautomerase--           15.1         
5494tyrosinase Alu(-)---- ----    104.6-        
5883tyrosinase-related protein 1--           +         
5504prostaglandin synthase-2/cyclooxygenase +-         ++-         
5514midkine-++       +   ++++   -    
5515melanocortin 1 receptor--           -      +- 
5886keratin K5--           -+        
5527integrin alpha 2 subunit-+  +        -++ +++     
553315-lipoxygenase--                  +- 
5538breast carcinoma-associated antigen--       -    ++   -    
5546proatate-specific antigen/kallikrein 3-+           -         
5548keratin 17--           +         
5907keratin 17-+           33.3 
5910proatate-specific antigen/kallikrein 3 +-         +++-



Endothelium
RDBhuman promoterHeLaHeLa +TNFalphaHepG2COS-7C2C12CHO-K1B16MCF-7F9F9 +RASaos-2HEK293K562K562 +TPA
5477endoglin- --17.4+  ----  
5506plasminogen activator inhibitor 1- 55.8           
5517integrin beta-3- --    -+- -+
5518integrin alpha v- ++     17.940.8  29.1++
5520I kappa B-alpha/(MAD3)--++     --    
5525receptor tyrosine kinase/vascular endotherial growth factor receptor- -     ++    
5526interleukin 1 beta- -     --  -22.2
5527integrin alpha 2 subunit- ++  -+++++    
5547urokinase plasminoden activtor- ++     ++-   



Nerve
RDBhuman promoterHeLaHeLa +20% FCSHepG2HepG2 +dexamethasoneHepG2 heat shockCOS-7C2C12C2C1 +DMC2C1 +2% horse serumPC12PC12 +NGFCHO-K1B16MCF-7K562K562 +TPA
5474bone morphogenetic protein 6- -      ++--   
5484elongation factor 1 A-2- -  -+- 11.0 ++     
5885proprotein convertase2- -       +++     
5519inhibitor of differentiation-237.343.4125.2151.0 13.6++++-++33.923.5  ++65.0 44.2
5536corticotropin releasing hormone receptor 1- -       +++  -  
5890myelin basic protein (MBP)- -      -+     



Genital
RDBhuman promoterHeLaHepG2CHO-K1NEC8LNCaPLNCaP +DexB16MCF-7MtT/SMF9F9 +RASaos-2HEK293NIH3T3
5466activin beta-A---+--- +  -- 
5884TR3 orphan receptor-+10.9+11.2 ++        
5500leukoprotease inhibitor++++-          
5546proatate-specific antigen/kallikrein 3-+    -       
5547urokinase plasminoden activtor-++       ++-  
5902placental-type alkaline phosphatase 535aa--+-         -
5903placental-like alkaline phosphatase 532aa--+372.4       -  
5910proatate-specific antigen/kallikrein 3 +-  +++-      



Cancer
RDBhuman promoterHeLaHepG2HepG2 +dexamethasoneCOS-7C2C12C2C12 +DMCHO-K1NEC8B16MCF-7F9F9 +RASaos-2Saos-2 serum starvationHEK293K562K562 +TPANIH3T3WI38
5467aldose reductase+++      +   - -   -
5477endoglin-- -17.4 +   --- -    
5479collagenase type IV-44.5  +-  +-  -    46.9 
5493protein tyrosine phosphatase 1B-10.4          -  97.0 92.9  
5514midkine-++    + ++++  -      
5516L-plastin-+       -  -  20.4103.0   
5517integrin beta-3-- -      -+-  -+  
5522telomerase RNA-56.4          -      
5528insulin-like growth factor II,P4 promoter-++          -      
5529p73 tumor protein--  -       --   + 
5532alpha-fetoprotein--++         -      
5534telomerase reverse transcriptase,catalytic subunit-+       -  - -    
5538breast carcinoma-associated antigen--    -  ++  -      
5547urokinase plasminoden activtor-++        ++-    
5903placental-like alkaline phosphatase 532aa--    +372.4    -    
5909protein tyrosine phosphatase 1B 475.51861.3          498.8  1302.7609.1



Cell cycle
RDBhuman promoterHeLaHepG2COS-7NIH3T3NIH3T3 serum starvation
5476cdc25A-+ --
5482cyclin A1+++ +-
5483transcription factor E2F1 -+ --
5508p27 cyclin-dependent kinase inhibitor++ ++
5510p14 alternative reding frame--+- 
5537transcription factor E2F1 -+ --
5893p21 cyclin dependenk kinase inhibitor/WAF1/CIP1-++ 23.313.2
5908cdc25c -- +-
5811p16 cyclin-dependent kinase inhibitor 2-+ - 



Leukocyte
RDBhuman promoterHeLaHeLa +20% FCSHepG2HepG2 +dexamethasoneHepG2 heat shockCOS-7C2C12C2C12 +DMC2C1 +2% horse serumPC12PC12 +NGFB16MCF-7F9F9 +RASaos-2K562K562 +TPANIH3T3
5472beta-2 microglobulin12.2 +++++ ++-    11.8   40.5205.1 
5478collagenase-1- +   33.2    11.3    ++++45.4
5481CXC chemokine SDF1 receptor- +             ++ 
5485early growth response protein 1- 342.1            ++702.71046 
5488Fas ligand- -             +- 
5493protein tyrosine phosphatase 1B- 10.4            -97.0 92.9 
5515melanocortin 1 receptor- -        -    +- 
5516L-plastin- +         -  -20.4103.0  
5517integrin beta-3- -  -       -+--+ 
5518integrin alpha v- ++          17.940.8 29.1++ 
5519inhibitor of differentiation-237.343.4125.2151.0 13.6++++-++33.923.5 ++   65.0 44.2 
5526interleukin 1 beta- -          -- -22.2 
5527integrin alpha 2 subunit- +  +     -+++++    
553315-lipoxygenase- -             +- 
5895L-selectin- -             12.7++ 
5909protein tyrosine phosphatase 1B 475.5 1861.3            498.81302.7609.1 



Others
RDBhuman promoterHeLaHepG2HepG2 heat shockCOS-7C2C12C2C12 +DMC2C12 +2% horse serumPC12CHO-K1LNCaPB16MCF-7MCF7 +progesteroneMtT/SMMIN7F9Saos-2HEK293K562NIH3T3WI38
5469alpha 6 integrin sudunit-++  --     +-        
5497transforming growth factor-beta type ‡Ureceptor-++                   
5498sterol regulatoty element-binding protein-2-+               +   
5507p53-15.2                   
5511proly 4-hydroxylase beta-subunit and disulfide isomerase+18.9                   
5531somatomammotropin-+           12.0 -    - 
5888Smad7-21.2 66.2             81.3   
554172-kDa heat shock protein10.2+-              10.5   
5912alpha 6 integrin sudunit -15.3         ++        
5914PAX3++++ ++      ++          
5915MSX2(Hox8)++42.8      15.4 65.3          
5812c-kit+11.1  18.2 18.4 ++ ++          
5549pKM2L-pvTK++     +  -   ++  -   -
5550pKM2L-pvSV40175.470.7 ++++++   +++++ +++++++++  ++++++++-++++++++-
5551pKM2L-pvCMV456.0 205.8        +++     +   +

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2009.02.20