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| Database |
Ordering Instructions (in Japanese)
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| Data Sheet |
| Library Name | # Plates | Clone Numbers | Funded By | Reference |
|---|---|---|---|---|
| 1-cell Unfertilized Egg | 61 | J0400A01 - J0460H12 | ERATO Doi | Development 127, 1737-1749 |
| 1-cell Fertilized Egg | 61 | J0200A01 - J0260H12 | ERATO Doi | Development 127, 1737-1749 |
| 2-cell Embryo | 51 | J0700A01 - J0750H12 | ERATO Doi | Development 127, 1737-1749 |
| 4-cell Embryo | 51 | J1000A01 - J1050H12 | ERATO Doi | Development 127, 1737-1749 |
| 8-cell Embryo | 51 | J0500A01 - J0550H12 | ERATO Doi | Development 127, 1737-1749 |
| 16-cell Embryo | 31 | J0800A01 - J0830H12 | ERATO Doi | Development 127, 1737-1749 |
| 16-cell Embryo | 51 | J0900A01 - J0950H12 | ERATO Doi | Development 127, 1737-1749 |
| 3.5-dpc Blastocyst | 111 | J0001A01 - J0111H12 | ERATO Doi | Development 127, 1737-1749 |
| Total Plates | 468 |
| Name of clones : | Preimplantation Mouse Embryonic EST clones |
| Comments : | cDNA clones isolated from cDNA libraries constructed using oligo-dT primer and mRNA purified from unfertilized egg, fertilized egg, 2 cell stage, 4 cell stage, 8 cell stage, 16 cell stage or blastcyst stage C57BL/6J embryos. Single pass sequence data from 3'-end have been available from dbEST data base. |
| Locus symbol : | D (Chromosome No.) Ertd (clone No.) e |
| Chromosome location : | (798 clones have been mapped by Feb. 99; data available at The Jackson Laboratory's Mouse Genome Informatics site (http://www.informatics.jax.org/) |
| Description of DNA insert : | mRNA purified from above-mentioned early mouse embryos was used to synthesize cDNA with oligo dT primer (Not I primer-adaptor from Gibco BRL). The double-stranded cDNA was ligated to Lone-linker LL-Sal3 and amplified by PCR. Following digestion with Sal I and Not I, the cDNA was size-selected by Size Fractionation Column (Gibco BRL). |
| Type of inserted DNA : | cDNA |
| Size of inserted DNA : | 1.5 kb on average |
| Vector : | pSPORT1 (Gibco BRL Life Technology) |
| Size of Vector : | 4,109 bp |
| Classification : | Plasmid |
| Cloning site excised by : | Sal I and Not I |
| Genetic markers : | ampicillinr |
| Host : | E. coli (DH10B) |
| Growth temperature : | 37 C |
| Medium and additives : | LB+ampicillin |
| Keyword : | pSPORT1, preimplantation mouse embryonic cDNA libraries, oligo dT primer, dbEST |
| Reference : | (Tentative reference; these will be replaced when full paper is published.) |
| J.R. Kitchen et al. (1997) Systematic Analyses of Genes Expressed in 3.5 dpc Mouse Blastcyst. Abstracts of 1997 meeting on Mouse Molecular Genetics; p128. M.S.H. Ko et al. (1998) Sequencing and Mapping of Genes Expressed in Mouse Embryos from Preimplantation Stages (from Unfertilized Egg to Blastcyst). Abstracts of 12th International Mouse Genome Conference; B26. | |
| Depositors : | Japan science and Technology Corporation and Wayne State University |
| Developers : | Dr. Hirofumi Doi and Dr. Minoru S. H. Ko |
| Restriction of distribution: | With Dr. Doi's written consent and acknowledgement to Dr. Doi in the publications. (c,y) |
| 使用上の注意: |
第二種使用等を目的とした遺伝子組換え生物です。 クローンの宿主は大腸菌です。 組換え核酸の名称は、各クローンの詳細情報をご参照下さい。 |
| 提供制限 : | 樹立者に許可をとること。研究結果の発表時、謝辞に樹立者名を入れるこ と。 (c, y) |
| 提供に必要な書類 : | 書式A, C, Dおよび所属機関発行の組換え実験承認書 |
| 提供申込書の送付先: | 理化学研究所 筑波研究所 企画課 FAX：029-836-9182 |
| 樹立者の連絡先: | 土居 洋文 |
これらの書式は、ホームページからダウンロードされるか、カタログ(送料着払いにて送付可)掲載の書式をご使用下さい。書式Dには、樹立者のサインが必要ですので、サインを頂いてから、こちらに御送り下さい。
dnabank@brc.riken.jp お問い合わせはこちらまで
2005.08.17