Gene Analysis in Mammalian Cell

• Plasmid/cosmid



RDB	Name of clone	Running title	Class	Seq file
1379	pH2Rneo	Expression vector with simian virus 40 (SV40) early promoter	Vector
1674	pCALNLw	A cassette to introduce cDNA into mammalian chromosome	Vector
1679	pCALwL	A cassette plasmid to insert cDNA into downstream CAG promoter	Vector
1862	pCALNL5	A cassette to introduce cDNA into mammalian chromosome	Vector
1968	pBMSA	Expression vector, inducible promoter	Vector
2478	pCEAGpolyA	mammalian expression vector driven by human CEA promoter	Vector
2546	pxCAG	Expression vector driven by CAG promoter	Vector
3043	pMNSM	Retrovirus expression vector in eukaryotic cells	Vector



• pCMV_tag vectors (ja)  pCMV_tag vectors (en)

RDB	Name of clone	Running title	Class	Seq file
2136	S-HA-pRc/CMV	Expressing HA-tagged fusion protein, CMV promoter	Vector	RDB2136.pdf
2137	S-T7-pRc/CMV	Expressing T7-tagged fusion protein, CMV promoter	Vector	RDB2137.pdf
2138	D-T7-pRc/CMV	Expressing a fusion protein tagged with duplicated T7, CMV promoter	Vector	RDB2138.pdf
2139	D-HA-pRc/CMV	Expressing a fusion protein tagged with duplicated HA, CMV promoter	Vector	RDB2139.pdf
2140	S-Myc-pRc/CMV	Expressing Myc-tagged fusion protein, CMV promoter	Vector	RDB2140.pdf
2141	D-Myc-pRc/CMV	Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter	Vector	RDB2141.pdf
5954	pCMV_S-Myc	Expressing Myc-tagged fusion protein, CMV promoter	Vector	RDB5954.pdf
5955	pCMV_D-Myc	Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter	Vector	RDB5955.pdf
5956	pCMV_S-FLAG	Expressing FLAG-tagged fusion protein, CMV promoter	Vector	RDB5956.pdf
Sequence of MCS (pdf)

• Adenovirus

RIKEN BRC DNA Bank provides hundreds of shuttle vectors to generate recombinant adenovirus. We also provide vectors to construct shuttle vector for recombinant adenovirus. A list of the vectors is available at
http://dna.brc.riken.jp/rvd/allavector.html

Vectors to construct shuttle vector for recombinant adenovirus.
Pdf file is available.




• Retrovirus



RDB no.	Name of vector	Short title	Classification
1699	pRx nZ ires Neo	Nls (nuclear localization signal), lacZ, ires, neo	Vector