Shuttle vectors for construction of recombinant adenovirus

RDB no.	Name of vector	Short title	Classification
917	pAxcw	A cassette cosmid for construction of recombinant adenovirus	Vector
918	pAxc	A cassette cosmid for construction of recombinant adenovirus	Vector
1678	pAxCAwt	A cassette cosmid for construction of a recombinant adenovirus	Vector
2564	pAxCEAwtR	Cloning vector to generate recombinant adenovirus driven by human CEA promoter	Vector
2565	pAxCEAwt	Cloning vector to generate recombinant adenovirus driven by human CEA promoter	Vector
3120	pAxcwit	A dual cassette for constructing recombinant adenovirus	Vector
3121	pAxCAwtit	A dual cassette for constructing recombinant adenovirus containing CAG promoter	Vector
3220	pAF16Rct	Cosmid clone of genomic fragment of adenovirus type 5	Vector
3380	pAxCAiLacZit	Shuttle vector to generate recombinant adenovirus harbouring E. coli LacZ	ShuttleVector
3444	pUAd5L	Shuttle vector to generate recombinant adenovirus	Vector
3445	pUAd5LCA	Shuttle vector to generate recombinant adenovirus	Vector
3446	pUAd5LCALNL	Shuttle vector to generate recombinant adenovirus	Vector
3447	pUAd5LCA	Shuttle vector to generate recombinant adenovirus	Vector
3448	pUAd5LCALNL	Shuttle vector to generate recombinant adenovirus	Vector
5212	pAxcwit2	A dual cassette for constructing recombinant adenovirus	Vector
5213	pAxCAwtit2	A dual cassette for constructing recombinant adenovirus	Vector
5214	pAxCALNLwtit2	A dual cassette for constructing recombinant adenovirus	Vector
5215	pAxEFwtit2	A dual cassette for constructing recombinant adenovirus	Vector

Name of vector	Cloning site	Promotera	Cre-loxPb	Production of recombinant adenovirus				RDB no.	Map
				Intact-genome transfectionc	Two-cosmidd	COS-TPCe	Fiber-modified COS-TPC
pAxcw	SwaI					Yes		917	[ pdf ]
pAxCAwt	SwaI	CA				Yes		1678	[ pdf ]
pAxCALNLw	SwaI	CA	Yes			Yes		f
pAxcwit	SwaI			Yes		Yes		3120	[ pdf ]
pAxCAwtit	SwaI	CA		Yes		Yes		3121	[ pdf ]
pUAd5L	SwaI				Yes	Yes	Yes	3444	[ pdf ]
pUAd5LCA	SwaI	CA			Yes	Yes	Yes	3445	[ pdf ]
pUAd5LCALNL	SwaI	CA	Yes		Yes	Yes	Yes	3446	[ pdf ]
pAxCEAwt	SwaI	CEA				Yes		2565	[ pdf ]
pAxcwit2	SwaI			Yesg		Yes		5212	[ pdf ]
pAxCAwtit2	SwaI	CA		Yesg		Yes		5213	[ pdf ]
pAxCALNLwtit2	SwaI	CA	Yes	Yesg		Yes		5214	[ pdf ]
pAxEFwtit2	SwaI	EF-1 alpha		Yesg		Yes		5215	[ pdf ]

a, CA, a modified promoter of the chicken gene for beta-actin with a cytomegalovirus-immediate early enhancer (CMV-IE); CEA, carcinoembryonic antigen; EF-1 alpha, elongation factor 1 alpha.

b, Target gene harbored in the recombinant adenovirus can be expressed by co-infection with AxCANCre (RDB no. 1748, Gene 181: 207-212, 1996).

c, Fukuda et al., Microbiol. Immunol. 50: 643-654, 2006.

d, Use pAF16Rct (RDB no. 3220) together to generate rAd.

e, Miyake et al., Proc. Natl. Acad. Sci. USA 93: 1320-1324, 1996.

f, Available from Takara Bio Inc.

g, PacI restriction enzyme is also applicable for linearization as well as Csp45I restriction enzyme.

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