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Plant DNA Catalogue>RIKEN Arabidopsis full-length cDNA clones (RAFL clones) 
This resource is called as RIKEN Arabidopsis full-length cDNA clones (RAFL clones) that has been developed by RIKEN Genomic Sciences Center (GSC) in Yokohama, Japan. The clones were deposited from Dr. Kazuo Shinozaki who is a director of RIKEN Plant Science Center (PSC) and a former group director of Plant Functional Genomics Research Group in RIKEN GSC. The RIKEN GSC, SSP (Salk/Stanford/PGEC) consortium and National Institute of Genetics (NIG) have characterized full-length sequence of approx. 18,000 RAFL clones with the supports from NSF 2010 Project and National BioResource Project. By the SSP consortium, the RAFL clones are called as ‘R clone’ and distinguished from ‘U clone’ that harbors ORF sequence of R clone in pUNI vector.
Resource number: pda00001- (with full-length sequence),
pdx00001-/pdy00001-/pdz00001- (with 5’-and/or 3’-end sequences)
Developer: Drs. Motoaki Seki and *Kazuo Shinozaki (RIKEN GSC, *RIKEN PSC).
Total number of clones: approx. 18,000 (pda), 220,000 (pdx/pdy/pdz)
Style of distribution:Approx. 100 ng of lyophilized plasmid DNA is provided.
Number of available clones: 16,915 clones (pda), 228,886 clones (pdx/pdy/pdz) (Mar. 2006)
Background ecotype: Columbia
Vector: modified pBluescript
Selection marker: ampicillin
Map and restriction site: :Shown here
Specific feature: Full-length sequence and/or 5’-/3’-end sequences have been characterized.
Specific conditions for distribution:
| (1) |
When the RECIPIENT has filed a patent application on the invention conceived or made during the course of research studies directly using the BIOLOGICAL RESOURCE, the RECIPIENT agrees to promptly notify RIKEN Plant Science Center (the owner of the BIOLOGICAL RESOURCE) upon filing the application with specifications. |
| (2) |
The RECIPIENT agrees not to use BIOLOGICAL RESOURCE for constructing microarray or macroarray without the agreement from the RIKEN PSC. |
| (3) |
The RECIPIENT can apply up to 100 clones of the BIOLOGICAL RESOURCE for distribution at once. |
| (4) |
The RECIPIENT agrees to expressly describe that “the BIOLOGICAL RESOURCE (the resource name) was developed by the plant genome project of RIKEN Genomic Sciences Center, and provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan” in Materials and Methods or in the Acknowledgement of any publication reporting the use thereof. The manuscript(s) listed below should be referred in the publication on scientific journal. The RECIPIENT also agrees to send a copy of such publication to the RIKEN BRC. The RIKEN BRC may disclose publicly, copy or otherwise use such publication to demonstrate the contribution by the RIKEN BRC. |
Reference:
| (1) |
Seki, M., Carninci, P., Nishiyama, Y., Hayashizaki, Y. and Shinozaki, K. (1998) Plant Journal 15: 707-720. PMID: 9778851 |
| (2) |
Seki, M., Narusaka, M., Kamiya, A., Ishida, J., Satou, M., Sakurai, T., Nakajima, M., Enju, A., Akiyama, K., Oono, Y., Muramatsu, M., Hayashizaki, Y., Kawai, J., Carninci, P., Itoh, M., Ishii, Y., Arakawa, T., Shibata, K., Shinagawa, A. and Shinozaki, K. (2002) Science 296: 141-145. PMID: 11910074 |
| (3) |
Sakurai T, Satou M, Akiyama K, Iida K, Seki M, Kuromori T, Ito T, Konagaya A, Toyoda T, and Shinozaki K. (2005) Nucleic Acids Res. 33(Database issue): D647-50. PMID: 15608280 |
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