| Resource number: pss00001-pss00009, developed by RIKEN BRC (The pss00009 contains 17 seed pools. Others contain 20 seed pools.); pss00101-00128, developed by RIKEN GSC (The pss00103 contains 16 seed pools. Others contain 20 seed pools.). |
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| Developer: Dr. Kazuo Shinozaki (Plant Molecular Biology Laboratory, RIKEN Tsukuba Institute), Dr Masatomo Kobayashi (RIKEN BRC), Dr. Minami Matsui (RIKEN GSC) |
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| Total number of line: approx. 70,000 |
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| Style of distribution: Provided as a seed pool set (seed pool contains approx. 400 seeds from 50 lines = 8 seeds per line; one seed pool set contains 20 seed pools = equivalent with 1,000 lines). Seeds for individual lines (psa and psm) that constitute specific seed pool (psp) are available for the users who obtained desired mutant in the seed pool. We also intend to distribute seeds for individual mutant lines (psa and psm) that showed interesting phenotype in near future. |
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| Number of pool sets available: 37 seed pool sets (equivalent with 36,650 lines) |
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| Background ecotype: Columbia |
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| Vector for transformation with Agrobacterium: pPCVICEn4HPT |
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| Selection marker: hygromycin |
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| Specific feature: Provided as seed pools for screening. The 4x35S enhancer in T-DNA promotes gene expression near T-DNA insertion site to produce dominant phenotype. |
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| Specific conditions for distribution: |
| (For pss00001-00009) |
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If the RECIPIENT claims any kind of intellectual property obtained from the use of the BIOLOGICAL RESOURCE, the RECIPIENT is required to notice RIKEN BRC after application. |
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| (For pss00101-00128) |
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When the RECIPIENT has filed a patent application on the invention conceived or made during the course of research studies directly using the BIOLOGICAL RESOURCE, the RECIPIENT agrees to promptly notify RIKEN Plant Science Center (the owner of the BIOLOGICAL RESOURCE) upon filing the application with specifications. |
| (for detail please send an e-mail to plant@brc.riken.jp) |
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| Reference: |
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Nakazawa M., Ichikawa T., Ishikawa A., Kobayashi H., Tsuhara Y., Kawashima M., Suzuki K., Muto S., Matsui M. (2003) Activation tagging, a novel tool to dissect the functions of a gene family. Plant Journal 34(5): 741-750. |
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| NOTE: Detail information of the activation-tagged line from RIKEN GSC is available from the website, http://rarge.gsc.riken.jp/. |
