| Resource number: pss11001, pss11002 |
|
| Developer: Dr. Minami Matsui (RIKEN PSC) |
|
| Total number of line: approx. 20,000 (expected) |
|
| Style of distribution: Provided as a seed pool set (seed pool contains approx. 400 seeds from 50 lines = 8 seeds per line; one seed pool set contains 20 seed pools = equivalent with 1,000 lines). Seeds for individual lines (psx) that constitute specific seed pool (psp) are available for the users who obtained desired mutant in the seed pool. |
|
| Number of pool sets available: 2 seed pool set (equivalent with 2,000 lines) |
|
| Background ecotype: Columbia |
|
| Vector for transformation with Agrobacterium: pBIG2113SF(pBI121 derivative) |
|
| Selection marker: hygromycin |
|
| Specific feature: Provided as seed pools for screening. Rice full-length cDNA was introduced with 35S promoter to produce dominant phenotype. |
|
| Specific conditions for distribution: |
| (1) |
The RECIPIENT agrees to expressly describe that “the BIOLOGICAL RESOURCE (the resource name) was developed by the RIKEN PSC, NIAS and RIBS through The Special Coordination Fund for Promoting Science and Technology, and provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan” in Materials and Methods or in the Acknowledgement of any publication reporting the use thereof. The RECIPIENT also agrees to send a copy of such publication to the RIKEN BRC. The RIKEN BRC, RIKEN PSC, NIAS and RIBS may disclose publicly, copy or otherwise use such publication to demonstrate the contribution by the RIKEN BRC. |
|
| Requirements for the safe handling, storage, transport and use: |
| (1) |
This BIOLOGICAL MATERIAL is the seed of Arabidopsis thaliana. |
| (2) |
This BIOLOGICAL MATERIAL harbor HPT gene (from E.coli), promoter of CaMV 35S RNA gene (from cauliflower mosaic virus), LB, RB and NOS-terminator sequences of T-DNA (Agrobacterium tumefaciens), and cDNA of rice (Oryza sativa). |
| (3) |
This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it for "Intentional introduction into the environment". This BIOLOGICAL MATERIAL and its progeny should be inactivated by autoclave or equivalent procedure before disposed as a waste. |
