| Resource number: pst00001-pst20808 |
|
| Developer: Dr Kazuo Shinozaki, Plant Functional Genomics Research Group, RIKEN GSC. |
|
| Total number of line: approx. 16,000 |
|
| Style of distribution: Approx. 50 seeds of individual line are provided. |
|
| Number of lines available: 15,267 lines |
|
| Background ecotype: No-0 (Nossen)* *Please read IMPORTANT NOTE (4) |
|
| Vector for transformation with Agrobacterium: pCGN derivatives |
|
| Selection marker: hygromycin |
|
| Specific feature: Flanking sequences of transposon have been |
|
| characterized. Sequences for Ds specific primers are here. |
|
| Specific conditions for distribution: |
| (1) |
When the RECIPIENT has filed a patent application on the invention conceived or made during the course of research studies directly using the BIOLOGICAL RESOURCE, the RECIPIENT agrees to promptly notify RIKEN Plant Science Center (the owner of the BIOLOGICAL RESOURCE) upon filing the application with specifications. |
| (2) |
The recipient can receive up to 50 lines of the BIOLOGICAL RESOURCE in each year. |
| (3) |
The RECIPIENT agrees to expressly describe that “the BIOLOGICAL RESOURCE (the resource name) was developed by the plant genome project of RIKEN Genomic Sciences Center, and provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan” in Materials and Methods or in the Acknowledgement of any publication reporting the use thereof. The manuscript(s) listed below should be referred in the publication on scientific journal. The RECIPIENT also agrees to send a copy of such publication to the RIKEN BRC. The RIKEN BRC may disclose publicly, copy or otherwise use such publication to demonstrate the contribution by the RIKEN BRC. |
|
| References: |
| 1. |
Fedoroff NV. and Smith D. (1993) A versatile system for detecting transposition in Arabidopsis. Plant J. 3: 273-289. |
| 2. |
Smith D., Yanai Y., Liu, Y-G., Ishiguro S., Okada K., Shibata D., Whittier RF. and Fedoroff NV. (1996) Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertional mutagenesis. Plant J. 10: 721-732. |
| 3. |
Ito T., Motohashi R., Kuromori T., Mizukado S., Sakurai T., Kanahara H., Seki M. and Shinozaki K. (2002) Plant Physiol. 129: 1695-1699. |
| 4. |
Kuromori T., Hirayama T., Kiyosue Y., Takabe H., Mizukado S., Sakurai T., Akiyama K., Kamiya A., Ito T. and Shinozaki K. (2004) Plant J. 37, 897-905. |
| 5. |
Ito T., Motohashi R., Kuromori T., Noutoshi Y., Seki M., Kamiya A., Mizukado S., Sakurai T. and Shinozaki K. (2005) Plant Cell Physiol. 46: 1149-1153. |
|
| IMPORTANT NOTE: |
| (1) |
AGI codes are frequently changed. Before you make your order, we recommend you to obtain detail information including flanking sequence of the line from RARGE (Riken Arabidopsis Genome Encyclopedia) or T-DNA express. |
| (2) |
The lines marked 'In preparation' are currently unavailable. Do not include those lines in your order. We will make an announcement at the time they are ready for distribution. |
| (3) |
The transposon-tagged lines are categorized to genetically modified organisms. For ordering such materials, the applicants must obtain all of the permission required by any kind of regulation or law before ordering. Also, it is recipient's responsibility to perform customs procedure for importation of biological materials. Please inform us when you need quarantine certification to import Arabidopsis seeds. Without providing such information, RIKEN BRC cannot guarantee any loss or damage of the materials that is caused during customs procedure. |
| (4) |
Genotypes of two Ds donor lines, namely Ds2-389-2 and Ds6-393-19, were inconsistent with that of Nossen. None of the natural accessions preserved in RIKEN BRC has same genotype with these lines. (These lines were crossed with Ac donor line to produce Ds12- and Ds16- lines.) We recommend users to use the parental lines available from ABRC as control plants in your experiments. List of parental line is here. |
|
| NOTE: Only those lines listed on our catalogue are available. Other lines will be open for distribution after multiplication in RIKEN BRC. For detail, please send your question to: plant@brc.riken.jp |
