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RIKEN BRC Mouse Phenome Database_ RMPD |
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- Protocols |
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Blood chemistry |
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- Mouse husbandry |
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| Ages |
Mice were housed in RIKEN BRC barrier facility until 8 weeks old when sample collection took place. |
| Diet |
All strains maintained on a Gamma-irradiated commercial diet (CLEA Rodent Diet CE-2, CLEA Japan, Inc., Tokyo, Japan) |
| Water |
Drinking water was supplied from a local source in pinhole type bottles (CLEA Japan, Inc., Tokyo, Japan) and autoclaved. |
| Housing |
Mouse R-PSF #CL-0132 cages were used for all strains as part of microisolation cage system (Safety Rack R, CLEA Japan, Inc., Tokyo, Japan). |
| Bedding |
Autoclaved paper bedding sheets, containing 75% soft and 25% hardwood pulps (CLEA Japan, Inc., Tokyo, Japan) |
| Photoperiod |
12 hrs on (covering the hrs of 8:00 to 20:00) 12hrs off. |
| Temperature |
23-25¡ëC |
| Relative humidity |
55-65% |
| Health status |
Mice were routinely monitored using the sentinel mouse program. See RIKEN BRC Quality Control website for the list of pathogenic agents monitored. |
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- Mouse information |
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| Sex: |
Both |
| Test age: |
8 weeks old |
| Pretreatment: |
Fasted for 4-5 hours |
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- Workflow |
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| Step |
Procedure accomplished |
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| 1 |
Whole blood collection |
Collected from each mouse using heparin-coated capillary micropipets via a retro-orbital bleed immediately prior to euthanasia. Stored at 4¡ëC over night. |
| 2 |
Blood plasma extraction |
Extracted by centrifuging at 13,000 rpm for 10 min. |
| 3 |
Blood plasma chemistry analysis |
Constituents of the blood chemistry panel included Cholesterol (CHOL), HDL Cholesterol (HDL), Triglyceride (TG-B), Amylase (AMY), Uric Acid (UA), Glucose (GLU), Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Total Bilirubin (TBIL), Calcium (CA), Creatinie (CREA), Blood Urea Nitrogen (BUN), Total Protein (TP), Albumin (ALB), Lactate Dehydrogenase (LD-L), Fe (FE), Serum Inorganic Phosphorus (PO4). |
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- Equipment |
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| Automated blood chemistry analyzer: Synchron CX5 delta (Beckman Coulter, Inc., Fullerton, CA, USA) |
| Capillary micropipets: Hematoclad Tubes Heparin Coated (Red) (Drummond Scientific company, Broomall, PA, USA) |
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- Data available |
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| Measurement abbreviation |
Description |
Ages (wks) |
Units |
| CHOL |
Plasma Cholesterol |
8 |
mg/dL |
| HDL |
Plasma HDL Cholesterol |
8 |
mg/dL |
| TG-B |
Plasma Triglyceride |
8 |
mg/dL |
| AMY |
Plasma Amylase |
8 |
U/L |
| UA |
Plasma Uric Acid |
8 |
mg/dL |
| GLU |
Plasma Glucose |
8 |
mg/dL |
| AST |
Plasma Aspartate Aminotransferase |
8 |
IU/L |
| ALT |
Plasma Alanine Aminotransferase |
8 |
IU/L |
| ALP |
Plasma Alkaline Phosphatase |
8 |
IU/L |
| TBIL |
Plasma Total Bilirubin |
8 |
mg/dL |
| CA |
Plasma Calcium |
8 |
mg/dL |
| CREA |
Plasma Creatinie |
8 |
mg/dL |
| BUN |
Plasma Blood Urea Nitrogen |
8 |
mg/dL |
| TP |
Plasma Total Protein |
8 |
g/dL |
| ALB |
Plasma Albumin |
8 |
g/dL |
| LD-L |
Plasma Lactate Dehydrogenase |
8 |
IU/L |
| FE |
Plasma Fe |
8 |
ug/dL |
| PO4 |
Plasma Serum Inorganic Phosphorus |
8 |
mg/dL |
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