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RIKEN BRC Mouse Phenome Database_ RMPD |
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- Protocols |
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Blood hematology |
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- Mouse husbandry |
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| Ages |
Mice were housed in RIKEN BRC barrier facility until 8 weeks old when sample collection took place. |
| Diet |
All strains maintained on a Gamma-irradiated commercial diet (CLEA Rodent Diet CE-2, CLEA Japan, Inc., Tokyo, Japan) |
| Water |
Drinking water was supplied from a local source in pinhole type bottles (CLEA Japan, Inc., Tokyo, Japan) and autoclaved. |
| Housing |
Mouse R-PSF #CL-0132 cages were used for all strains as part of microisolation cage system (Safety Rack R, CLEA Japan, Inc., Tokyo, Japan). |
| Bedding |
Autoclaved paper bedding sheets, containing 75% soft and 25% hardwood pulps (CLEA Japan, Inc., Tokyo, Japan) |
| Photoperiod |
12 hrs on (covering the hrs of 8:00 to 20:00) 12hrs off. |
| Temperature |
23-25ˇëC |
| Relative humidity |
55-65% |
| Health status |
Mice were routinely monitored using the sentinel mouse program. See RIKEN BRC Quality Control website for the list of pathogenic agents monitored. |
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- Mouse information |
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| Sex: |
Both |
| Test age: |
8 weeks old |
| Pretreatment: |
None |
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- Workflow |
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| Step |
Procedure accomplished |
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| 1 |
Blood collection with EDTA anticoagulant |
Collected from each mouse using capillary micropipets via a retro-orbital bleed immediately prior to euthanasia. |
| 2 |
hematology analysis |
Constituents of the blood chemistry panel included White Blood Cell (WBC), Red Blood Cell (RBC), Hemoglobin (HBG), Hematocrit (HCT), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Platelet (PLT). |
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- Equipment |
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| hematology analyzer: Celltac¦Á MEK-6358 (NIHON KOHDEN COPARATION, Tokyo, Japan) |
| Capillary micropipets: Hematoclad Tubes Plain (Blue) (Drummond Scientific company, Broomall, PA, USA) |
| Blood collection tube: Capiject Micro Collection Tubes (#CJ-NA, TERUMO COPARATION, Tokyo, Japan) |
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- Data available |
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| Measurement abbreviation |
Description |
Ages (wks) |
Units |
| WBC |
White Blood Cell |
8 |
10^2/μL |
| RBC |
Red Blood Cell |
8 |
10^4/μL |
| HGB |
Hemoglobin |
8 |
g/dL |
| HCT |
Hematocrit |
8 |
% |
| MCV |
Mean Corpuscular Volume |
8 |
fL |
| MCH |
Mean Corpuscular Hemoglobin |
8 |
pg |
| MCHC |
Mean Corpuscular Hemoglobin Concentration |
8 |
g/dL |
| PLT |
Platelet |
8 |
10^4/μL |
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