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RIKEN BRC Mouse Phenome Database_ RMPD
- Protocols
Blood chemistry
- Mouse husbandry
Ages Mice were housed in RIKEN BRC barrier facility until 8 weeks old when sample collection took place.
Diet All strains maintained on a Gamma-irradiated commercial diet (CLEA Rodent Diet CE-2, CLEA Japan, Inc., Tokyo, Japan)
Water Drinking water was supplied from a local source in pinhole type bottles (CLEA Japan, Inc., Tokyo, Japan) and autoclaved.
Housing Mouse R-PSF #CL-0132 cages were used for all strains as part of microisolation cage system (Safety Rack R, CLEA Japan, Inc., Tokyo, Japan).
Bedding Autoclaved paper bedding sheets, containing 75% soft and 25% hardwood pulps (CLEA Japan, Inc., Tokyo, Japan)
Photoperiod 12 hrs on (covering the hrs of 8:00 to 20:00) 12hrs off.
Temperature 23-25C
Relative humidity 55-65%
Health status Mice were routinely monitored using the sentinel mouse program. See RIKEN BRC Quality Control website for the list of pathogenic agents monitored.

- Mouse information
Sex: Both
Test age: 8 weeks old
Pretreatment: Fasted for 4-5 hours

- Workflow
Step Procedure accomplished  
1 Whole blood collection Collected from each mouse using heparin-coated capillary micropipets via a retro-orbital bleed immediately prior to euthanasia. Stored at 4C over night.
2 Blood plasma extraction Extracted by centrifuging at 13,000 rpm for 10 min.
3 Blood plasma chemistry analysis Constituents of the blood chemistry panel included Cholesterol (CHOL), HDL Cholesterol (HDL), Triglyceride (TG-B), Amylase (AMY), Uric Acid (UA), Glucose (GLU), Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Total Bilirubin (TBIL), Calcium (CA), Creatinie (CREA), Blood Urea Nitrogen (BUN), Total Protein (TP), Albumin (ALB), Lactate Dehydrogenase (LD-L), Fe (FE), Serum Inorganic Phosphorus (PO4).

- Equipment
Automated blood chemistry analyzer: Synchron CX5 delta (Beckman Coulter, Inc., Fullerton, CA, USA)
Capillary micropipets: Hematoclad Tubes Heparin Coated (Red) (Drummond Scientific company, Broomall, PA, USA)

- Data available
Measurement abbreviation Description Ages (wks) Units
CHOL Plasma Cholesterol 8 mg/dL
HDL Plasma HDL Cholesterol 8 mg/dL
TG-B Plasma Triglyceride 8 mg/dL
AMY Plasma Amylase 8 U/L
UA Plasma Uric Acid 8 mg/dL
GLU Plasma Glucose 8 mg/dL
AST Plasma Aspartate Aminotransferase 8 IU/L
ALT Plasma Alanine Aminotransferase 8 IU/L
ALP Plasma Alkaline Phosphatase 8 IU/L
TBIL Plasma Total Bilirubin 8 mg/dL
CA Plasma Calcium 8 mg/dL
CREA Plasma Creatinie 8 mg/dL
BUN Plasma Blood Urea Nitrogen 8 mg/dL
TP Plasma Total Protein 8 g/dL
ALB Plasma Albumin 8 g/dL
LD-L Plasma Lactate Dehydrogenase 8 IU/L
FE Plasma Fe 8 ug/dL
PO4 Plasma Serum Inorganic Phosphorus 8 mg/dL
 
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