RIKEN BRC Mouse Phenome Database_ RMPD
- Protocols
Blood hematology
- Mouse husbandry
Ages Mice were housed in RIKEN BRC barrier facility until 8 weeks old when sample collection took place.
Diet All strains maintained on a Gamma-irradiated commercial diet (CLEA Rodent Diet CE-2, CLEA Japan, Inc., Tokyo, Japan)
Water Drinking water was supplied from a local source in pinhole type bottles (CLEA Japan, Inc., Tokyo, Japan) and autoclaved.
Housing Mouse R-PSF #CL-0132 cages were used for all strains as part of microisolation cage system (Safety Rack R, CLEA Japan, Inc., Tokyo, Japan).
Bedding Autoclaved paper bedding sheets, containing 75% soft and 25% hardwood pulps (CLEA Japan, Inc., Tokyo, Japan)
Photoperiod 12 hrs on (covering the hrs of 8:00 to 20:00) 12hrs off.
Temperature 23-25ˇëC
Relative humidity 55-65%
Health status Mice were routinely monitored using the sentinel mouse program. See RIKEN BRC Quality Control website for the list of pathogenic agents monitored.

- Mouse information
Sex: Both
Test age: 8 weeks old
Pretreatment: None

- Workflow
Step Procedure accomplished  
1 Blood collection with EDTA anticoagulant Collected from each mouse using capillary micropipets via a retro-orbital bleed immediately prior to euthanasia.
2 hematology analysis Constituents of the blood chemistry panel included White Blood Cell (WBC), Red Blood Cell (RBC), Hemoglobin (HBG), Hematocrit (HCT), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Platelet (PLT).

- Equipment
hematology analyzer: Celltac¦Á MEK-6358 (NIHON KOHDEN COPARATION, Tokyo, Japan)
Capillary micropipets: Hematoclad Tubes Plain (Blue) (Drummond Scientific company, Broomall, PA, USA)
Blood collection tube: Capiject Micro Collection Tubes (#CJ-NA, TERUMO COPARATION, Tokyo, Japan)

- Data available
Measurement abbreviation Description Ages (wks) Units
WBC White Blood Cell 8 10^2/μL
RBC Red Blood Cell 8 10^4/μL
HGB Hemoglobin 8 g/dL
HCT Hematocrit 8 %
MCV Mean Corpuscular Volume 8 fL
MCH Mean Corpuscular Hemoglobin 8 pg
MCHC Mean Corpuscular Hemoglobin Concentration 8 g/dL
PLT Platelet 8 10^4/μL
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